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18 - Improving oocyte maturation in vitro
- from Section 3 - Developmental biology
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- By Jeremy G. Thompson, Research Centre for Reproductive Health, Robinson Institute, School of Paediatrics and Reproductive Health,The University of Adelaide, Adelaide, Australia, Robert B. Gilchrist, Research Centre for Reproductive Health, Robinson Institute, School of Paediatrics and Reproductive Health, The University of Adelaide, Adelaide, Australia
- Edited by Alan Trounson, Roger Gosden, Ursula Eichenlaub-Ritter, Universität Bielefeld, Germany
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- Book:
- Biology and Pathology of the Oocyte
- Published online:
- 05 October 2013
- Print publication:
- 24 October 2013, pp 212-223
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Summary
Introduction
Successful infertility treatment, especially in vitro fertilization (IVF) and accompanying clinical and laboratory technologies, has to be one of the great medical success stories of the late twentieth and early twenty-first centuries. In the space of 35 years of development, assisted reproduction through IVF now contributes up to 4% of all births in developed nations, with these figures set to increase further due to the influence of increasing maternal age to first conception, lifestyle choices, and the exposure to environmental toxins. The success of current technology utilized in an IVF cycle is dependent on gonadotropic hormone hyperstimulation of the ovary to generate large numbers of mature oocytes (Figure 18.1). Nevertheless, hormonal stimulation of the ovary by follicle stimulating hormone (FSH), or an analog, is associated with various risks and financial costs. These include: a health risk to women caused by severe ovarian hyperstimulation syndrome (OHSS), which affects 0.5–5% of gonadotropin-treated women [1]; a risk to oocyte and resulting embryo health (e.g., embryo an-euploidy [2] and perturbed genomic imprinting [3]); and a significant financial burden placed on couples and/or healthcare providers due to the cost of the gonadotropin treatment. Furthermore, FSH has dose-dependent adverse effects on subsequent embryo quality, endometrial protein expression, and pregnancy rates in adult, cycling female mice [4, 5]. Therefore, safe, reliable alternatives to the current clinical IVF practices that remove the need for hyperstimulatory FSH treatment, accompanied with increased treatment options for women, is highly attractive. This would be particularly so for women who suffer from the very prevalent condition of polycystic ovaries (estimated to be around 20% of women of reproductive age), who are particularly susceptible to OHSS and as a result, at times have limited IVF treatment options.
Chapter 17 - Current status and future trends of the clinical practice of human oocyte in vitro maturation
- Edited by David K. Gardner, University of Melbourne, Botros R. M. B. Rizk, University of South Alabama, Tommaso Falcone
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- Book:
- Human Assisted Reproductive Technology
- Published online:
- 16 May 2011
- Print publication:
- 31 March 2011, pp 186-198
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Summary
This chapter focuses on the non-invasive approaches which largely concentrate on proteomics analyses. Proteins are generated from the genome with signal sequences that direct them to the cell membrane or are secreted in the extracellular environment. Secreted proteins are often post-translationally modified, in particular by glycosylation. These proteins are then released into the interstitial environment where they may enter body fluids such as the blood system and, in the case of endometriosis, be detected in endometrial, peritoneal, or follicular fluids. In most proteomic approaches, mass spectrometry (MS) plays a part in the analyses. Electrospray ionization (ESI) is the most common method for sample introduction into the MS. Matrix assisted laser desorption ionization (MALDI) is also be used as an alternative to ESI. Two-dimensional gel analyses have traditionally been used for the differential analysis of proteomes and differences may be observed and selected visually or using image analysis of scanned gels.
Contributors
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- By Ashok Agarwal, Carrie Bedient, Nick Brook, Michelle Catenacci, Ying Cheong, Francisco Domínguez, Thomas Elliott, Sandro C. Esteves, Tommaso Falcone, Gabriel de la Fuente, Eugene Galdones, Juan A. Garcia-Velasco, David K. Gardner, Tamara Garrido, Robert B. Gilchrist, Georg Griesinger, Roy Homburg, Jeanine Cieslak Janzen, Mark T. Johnson, Jennifer Kahn, David L. Keefe, Efstratios M Kolibianakis, Laurie J. McKenzie, Nick Macklon, David Meldrum, Ashley R. Mott, Tetsunori Mukaida, Zsolt Peter Nagy, Edurne Novella-Maestre, Chris O’Neill, Chikaharo Oka, Steven F. Palta, Lewis K. Pannell, Antonio Pellicer, Valeria Pugni, Botros R. M. B. Rizk, Christopher B. Rizk, Claude Robert, Denny Sakkas, Hassan N. Sallam, William B. Schoolcraft, Lonnie D. Shea, Carlos Simón, Manuela Simoni, Marc-Andre Sirard, Johan E. J. Smitz, Eric S. Surrey, Jan Tesarik, Raquel Mendoza Tesarik, Jeremy G. Thompson, Andrew J. Watson, Teresa K. Woodruff
- Edited by David K. Gardner, University of Melbourne, Botros R. M. B. Rizk, University of South Alabama, Tommaso Falcone
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- Book:
- Human Assisted Reproductive Technology
- Published online:
- 16 May 2011
- Print publication:
- 31 March 2011, pp ix-xii
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Contributors
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- By Joanne R. Adler, David A. Alexander, Laurence Alison, Catherine C. Ayoub, Peter Banister, Anthony R. Beech, Amanda Biggs, Julian Boon, Adrian Bowers, Neil Brewer, Eric Broekaert, Paula Brough, Jennifer M. Brown, Kevin Browne, Elizabeth A. Campbell, David Canter, Michael Carlin, Shihning Chou, Martin A. Conway, Claire Cooke, David Cooke, Ilse Derluyn, Robert J. Edelmann, Vincent Egan, Tom Ellis, Marie Eyre, David P. Farrington, Seena Fazel, Daniel B. Fishman, Victoria Follette, Katarina Fritzon, Elizabeth Gilchrist, Nathan D. Gillard, Renée Gobeil, Agnieszka Golec de Zavala, Jane Goodman-Delahunty, Lynsey Gozna, Don Grubin, Gisli H. Gudjonsson, Helinä Häkkänen-Nyholm, Guy Hall, Nathan Hall, Roisin Hall, Sean Hammond, Leigh Harkins, Grant T. Harris, Camilla Herbert, Robert D. Hoge, Todd E. Hogue, Clive R. Hollin, Lorraine Hope, Miranda A. H. Horvath, Kevin Howells, Carol A. Ireland, Jane L. Ireland, Mark Kebbell, Michael King, Bruce D. Kirkcaldy, Heidi La Bash, Cara Laney, William R. Lindsay, Elizabeth F. Loftus, L. E. Marshall, W. L. Marshall, James McGuire, Neil McKeganey, T. M. McMillan, Mary McMurran, Joav Merrick, Becky Milne, Joanne M. Nadkarni, Claire Nee, M. D. O’Brien, William O’Donohue, Darragh O’Neill, Jane Palmer, Adria Pearson, Derek Perkins, Devon L. L. Polaschek, Louise E. Porter, Charlotte C. Powell, Graham E. Powell, Martine Powell, Christine Puckering, Ethel Quayle, Vernon L. Quinsey, Marnie E. Rice, Randall Richardson-Vejlgaard, Richard Rogers, Louis B Schlesinger, Carolyn Semmler, G. A. Serran, Ralph C. Serin, John L. Taylor, Max Taylor, Brian Thomas-Peter, Paul A. Tiffin, Graham Towl, Rosie Travers, Arlene Vetere, Graham Wagstaff, Helen Wakeling, Fiona Warren, Brandon C. Welsh, David Wexler, Margaret Wilson, Dan Yarmey, Susan Young
- Edited by Jennifer M. Brown, London School of Economics and Political Science, Elizabeth A. Campbell, University of Glasgow
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- Book:
- The Cambridge Handbook of Forensic Psychology
- Published online:
- 06 July 2010
- Print publication:
- 29 April 2010, pp xix-xxiii
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